Figure 1
From: Structure and characterization of a novel chicken biotin-binding protein A (BBP-A)
SDS-PAGE based analysis of the thermal stability of the tetramers of BBP-A. Samples saturated with BTN (+Biotin) prior to analysis as well as samples without added BTN (-Biotin) are shown. (a) bBBP-A, (b) bBBP-A in the presence of BTN and (c) bBBP-A(A74S) in the absence and presence of BTN. After acetylation in vitro, the samples were subjected to thermal treatment in SDS-PAGE sample buffer containing 2-mercaptoethanol and SDS. The different temperatures used in the analysis are indicated in the upper part of the figure (°C). The molecular weights of the standard proteins (Bio-Rad) are shown on the left.