Figure 11
From: Structure and characterization of a novel chicken biotin-binding protein A (BBP-A)
Fluorescence spectroscopy analysis of BBP-A and AVD. (a) Emission spectra of BBP-A in the absence of ligand (black) and with BTN (green), BSO (blue) or D-biotin sulfone (red) are shown. (b) Emission spectra of AVD. (c) Dissociation of D-biotin sulfone from BBP-A displaced with excess of BTN at 40°C. The measured emission intensity is plotted over time and corrected using the emission decay data from control measurements, which were made for protein saturated with BTN. Excitation at 280 nm was used.