Figure 4
From: Structure and characterization of a novel chicken biotin-binding protein A (BBP-A)
Immunological cross-reactivity of chicken BBP-A and AVD. (a) Western blot analysis of 1/100, 1/10 and 1/1 diluted samples of chicken AVD (10 μg) and undiluted bBBP-A protein (10 μg). The polyclonal antibody TdaVII against AVD [51] was used as the primary antibody. (b) The same analysis as in A using another polyclonal antibody (University of Oulu, Finland). (c) Dot-blot analysis of BBP-A (10 μg) produced in bacteria 'b' and in insect cells 'i'. The polyclonal antibody TdaVII against AVD was used as the primary antibody. Serial dilutions of AVD (10 μg) were used as controls (the dilution factors are indicated in the figure).