Figure 9

Biotin dissociation analysis. The dissociation of [³H]biotin from various proteins was determined over time. The individual dissociation rates of [³H]biotin measured for bBBP-A (black triangles), bBBP-A(A74S) (grey triangles), bBBP-A(T118F) (white triangles) and iBBP-A (black circles) are shown. Solid lines represent linear fit to data sets. Data measured previously for AVR2 (black rectangles), AVR6 (white rectangles) and AVR4 (white tilted rectangles), all produced in bacteria, are also shown [24, 28]. Data measured for chicken AVD (tilted black rectangles) are presented, too [42]. AVR6 and AVR4 carried the mutations C58S and C122S, respectively, which prevented the formation of intersubunit disulphide bridges [33]. The dissociation rates for streptavidin are shown as a dotted line according to [29].