Structure and characterization of a novel chicken biotin-binding protein A (BBP-A)

BMC Structural Biology

Table 1 Biochemical properties of different BBP-A forms.

	Gel filtration				SDS-PAGE-based thermostability assay		Dissociation of fluorescent BTN (25°C)		DSC
	Elution time (min)		Molecular mass (kDa)^a		T_r (°C)		k_diss (10^-5 s^-1)	Release 1 h (%)	T_m (°C)
	-BTN	+BTN	-BTN	+BTN	-BTN	+BTN			-BTN	+BTN
bBBP-A	29.9	30.1	42.7	40.9	N. D.	70	54.8	86	N. A.	103.4
bBBP-A (A74S)	30.0	30.1	41.9	40.8	N. D.	75	47.1	83	N. A.	103.2
bBBP-A (T118F)	30.1	29.9	41.3	42.7	N. D.	70	69.1	94	N. A.	102.3
iBBP-A	28.4	28.4	60.8	59.7	N. D.	75	20.4	53	N. M.	N. M.
AVD	28.2	28.2	62.5	63.6	60^b	90^b	2.0	10	83.5^c	117.0
bAVD	28.8	28.8	55.3	55.1	60^b	90^b	1.1	5	N. M.	N. M.

N. D. Protein appeared mainly in monomeric form already at room temperature; N. A. Not applicable; N. M. Not measured.
^aMolecular mass calculated from the measured elution times of thyroglobulin (670 kDa), gamma-globulin (158 kDa), BSA (67 kDa), ovalbumin (44 kDa) and myoglobin (17 kDa).
^bResults from ref. [30].
^cResults from ref. [33].

ISSN: 1472-6807