Novel structural features in two ZHX homeodomains derived from a systematic study of single and multiple domains

Bird, Louise E; Ren, Jingshan; Nettleship, Joanne E; Folkers, Gert E; Owens, Raymond J; Stammers, David K

doi:10.1186/1472-6807-10-13

BMC Structural Biology

Table 1 Expression constructs for human ZHXs

From: Novel structural features in two ZHX homeodomains derived from a systematic study of single and multiple domains

Protein	Construct OPPF_	Domain/s	aa range	5' primer	3' primer	Vector	Tag
ZHX1	1610	Full-length	1-874	ATGGCAAGCAGGCGAAAATCAAC	TCAGTCATCTGATTTAGACAGCTTCCG	pDEST14	N-His
	1611	HD1	284-343	AATAGCATTCCCACCTACAATGCTGC	CCAACTAACACCATGTTTTAAACGTTGGGC	pDEST14	N-His
	1612	HD3	556-633	CAGCCTAAGCAATCCTGGAATCC	ATCTATTTCCATTTTCTCTTCCTTTAAAGCTTTTG	pDEST14	N-His
	1613	HD4	655-731	GCACCTAAGTCAGGGAGTACAGGCAAG	TGAATTGGCGCTCTGATAGTAGTAGTACCATTTC	pDEST14	N-His
	1614	HD5	770-824	GACAGGGGACCATCACTCATAAAATTTAAAAC	TCTTCTCTGTCTTTCTGCAAACCACTCTCTG	pDEST14	N-His
	1615	HD3-4	556-731	CAGCCTAAGCAATCCTGGAATCC	TGAATTGGCGCTCTGATAGTAGTAGTACCATTTC	pDEST14	N-His
	1616	HD1-5	284-824	AATAGCATTCCCACCTACAATGCTGC	TCTTCTCTGTCTTTCTGCAAACCACTCTCTG	pDEST14	N-His
	1632	2ZF	60-153	AATCAGCAAAATAAAAAAGTTGAAGG	AAAAGTCAGATCATTTATTGTTTGTTC	pDEST14	N-His
	1633	2ZF	55-156	TCTGTGGATTCAGACAATCAGC	ACTACCATCAAAAGTCAGATCATTTATTG	pDEST14	N-His
	1634	HD2	464-523	TCATTTGGCATTCGGGCAAAAAAGAC	CTTTGAATTTCTCTGGTTGTACCTTGTG	pDEST14	N-His
	1635	HD2-3	464-633	TCATTTGGCATTCGGGCAAAAAAGAC	ATCTATTTCCATTTTCTCTTCCTTTAAAGCTTTTG	pDEST14	N-His
	1636	HD2-4	464-731	TCATTTGGCATTCGGGCAAAAAAGAC	TGAATTGGCGCTCTGATAGTAGTAGTACC	pDEST14	N-His
	1637	HD2-5	464-824	TCATTTGGCATTCGGGCAAAAAAGAC	TCTTCTCTGTCTTTCTGCAAACCACTCTCTG	pDEST14	N-His
	2264	HD3	556-633	CAGCCTAAGCAATCCTGGAATCC	ATCTATTTCCATTTTCTCTTCCTTTAAAGCTTTTG	pOPINF	N-His -3C
	2265	HD4	655-731	TCTGTGGATTCAGACAATCAGC	TTCTATACCTAATTCTGATCTTCTCTGTC	pOPINF	N-His -3C
	2465	All	55-830	TCTGTGGATTCAGACAATCAGC	TTCTATACCTAATTCTGATCTTCTCTGTC	pOPINF	N-His -3C
ZHX2	2268	2ZF	60-158	GTGATAGAGGTGAAATCTATGGG	GGTTTCGATGGACTGTTCCAAG	pDEST14	N-His
	2269	HD1	267-321	TACAACTCTGCCCTGGATACAA	CCAGCTGATGCCATGCTTTAAG	pDEST14	N-His
	2270	HD2	444-500	CGCAAGAAGACAAAGGAGCAG	GTGGACGATGCCCCTTTGAC	pDEST14	N-His
	2271	HD3	530-582	CCCCAGAAGTTCAAAGAGAAAAC	CTTCCGCCTCTCCGAGAACC	pDEST14	N-His
	2272	HD4	635-686	AAAAGTCAAGAACAGGTTCATCTC	TCCCGTTTTCAGCAAGCATCTG	pDEST14	N-His
	2273	HD2-3	444-582	CGCAAGAAGACAAAGGAGCAGA	CTTCCGCCTCTCCGAGAACC	pDEST14	N-His
	2466	All	55-745	TCCAAAGAAAACGAAGTGATAGAGGTGAAATC	CAACTTCTCCAAGTCCTCTTCGCAG	pOPINF	N-His -3C
ZHX3	2274	2ZF	66-153	AATGGGCATCGGAGCACTTTAG	CTGCTCCACAACCACATGATTG	pDEST14	N-His
	2275	HD1	306-363	ATTCCAACGTACAATGCAGCC	CCAGCTGATCCCCTGCTTCAG	pDEST14	N-His
	2276	HD2	500-553	AAGAAATCTCATGAACAGCTGTCAG	CTTCAAGTTCCGGCAGTGGTATC	pDEST14	N-His
	2277	HD3	621-664	CCTGAGCAGCTCAGAGCC	CCGTCTCTCTGAAAACCAGC	pDEST14	N-His
	2278	HD4	758-826	AAACTGGCAGAGCAGCTCC	CCATTTGAGTTGGCCGTTCTTC	pDEST14	N-His
	2279	HD2-3	500-664	AAGAAATCTCATGAACAGCTGTCAG	CCGTCTCTCTGAAAACCAGC	pDEST14	N-His
	2467	All	61-966	GGCTCTACACTGGCCAATGGGC	AGGTAAAGGAAAGGTCCTACATTGTGGG	pOPINF	N-His -3C

For the primers only the regions homologous to the target sequence are shown. 5' primers had GGGGACAAGTTTGTAC
CCAAAAAAGCAGGCTTCGAAGGAGATAGAACCATGGCACATCACCACCACCATCAC and AAGTTCTGTTTCAGGGCCCG 5' extensions, while 3' primers had GGGG
ACCACTTTGTACAAGAAAGCTGGGTCTCA and ATGGTCTAGAAAGCTTTA 5' extensions for cloning into pDEST14 or pOPINF vectors using either Gateway technology or the Infusion cloning system respectively.

Back to article page

ISSN: 1472-6807

Contact us

General enquiries: ORSupport@springernature.com