The intriguing Cyclophilin A-HIV-1 Vpr interaction: prolyl cis/trans isomerisation catalysis and specific binding

Table 1 Estimated kinetic constants for binding of HIV-1 N-terminal Vpr peptides to CypA.

Peptide	Amino acid sequence	Kinetic constants by two state reaction model
		K _D (M)	ka ₁ (1/Ms) ka ₂ (1/s)	kd(1/s)
s Vpr^21-40	ELLEELKSEAVRHFP³⁵ RIWLH	2.01 × 10 ^-4	k_a1 = 466 k_a2 = 0.0084	k_d1 = 0.164 k_d2 = 0.011
s Vpr^25-40	ELKSEAVRHFP³⁵ RIWLH	4.04 × 10 ^-4	k_a1 = 2004 K_a2 = 0.0028	k_d1 = 0.9415 k_d2 = 0.0173
s Vpr^1-40	MEQAP⁵EDQGP¹⁰QREP¹⁴YNEWTTLELLEELKSEAVRHFP³⁵ RIWLH	2.84 × 10 ^-4	k_a1 = 752 k_a2 = 0.0073	k_d1 = 0.37 k_d2 = 0.0099
s Vpr^1-40 P5,10,14 N	MEQAN⁵EDQGN¹⁰QREN¹⁴YNEWT TLELLEELKSEAVRHFP³⁵ RIWLH	0.00615*	k_a1 = 46* k_a2 = 0.0047	k_d1 = 0.56* k_d2 = 0.0048
s Vpr^30-40	AVRHFP³⁵ RIWLH	1.67 × 10 ^-4	k_a1 = 2467 k_a2 = 0.00145	k_d1 = 0.527 k_d2 = 0.0053
s Vpr^32-38	RHFP³⁵ RIW	4.87 × 10 ^-4	k_a1 = 924 k_a2 = 0.0011	k_d1 = 0.67 k_d2 = 0.0023
s Vpr^33-37	HFP³⁵RI	0.00357	k_a1 = 120.4 k_a2 = 0.00138	k_d1 = 0.66 k_d2 = 0.0025

ISSN: 1472-6807