Figure 6
From: Human Nek6 is a monomeric mostly globular kinase with an unfolded short N-terminal domain
Analytical size-exclusion chromatography of recombinant hNek6 variants fused to a 6xHis tag. (A) Chromatographic profile of hNek6 variants (colored lines) and a mixture of standard proteins (dashed line). (B) Linear fit of known Stokes radii (Rs) as a function of the measured partition coefficient (Kav). The colored circles show the partition coefficient of hNek6 variants, and the black squares, the partition coefficients of the standards. The circles colors correspond to the same colors depicted in (A). The proteins were eluted in 50 mM HEPES pH 7.5, 5 mM sodium phosphate, 600 mM NaCl, 5% glycerol, as homogeneous species at different elution volumes (Ve) using an analytical Superdex 200 10/300 GL column. Conalbumin, ovalbumin, carbonic anhydrase and ribonuclease were used as Stokes radii standards. (wt: wild-type, D: dephosphorylated).