Gα16 interacts with tetratricopeptide repeat 1 (TPR1) through its β3 region to activate Ras independently of phospholipase Cβ signaling

Table 4 Gα_16/z chimera mediated STAT3-driven and NFκB-driven luciferase activities.

*Construct*	*STAT3-driven Luciferase Fold Stimulation*	*NFκB-driven Luciferase Fold Stimulation*
pcDNA1	1.0 ± 0.0	1.1 ± 0.1
Gα₁₆	2.4 ± 0.1*	3.0 ± 0.2*
N30	1.7 ± 0.2*	2.1 ± 0.2*
N102	0.9 ± 0.1	1.3 ± 0.1
N155	1.0 ± 0.0	1.3 ± 0.0
N188	1.9 ± 0.2*	1.9 ± 0.2*
N200	1.5 ± 0.0*	1.7 ± 0.1*
N210	1.2 ± 0.1	1.2 ± 0.1
N246	0.9 ± 0.1	1.2 ± 0.1
N266	1.1 ± 0.1	1.2 ± 0.1
N295	1.2 ± 0.2	1.1 ± 0.1
C25	1.8 ± 0.1*	2.0 ± 0.1*
C44	1.6 ± 0.0*	1.9 ± 0.2*
C164	1.2 ± 0.0	1.3 ± 0.2
C174	1.2 ± 0.1	1.2 ± 0.1
C186	1.2 ± 0.0	1.3 ± 0.1
C219	1.3 ± 0.2	1.2 ± 0.1
C272	1.1 ± 0.1	1.2 ± 0.1
zβ3	1.7 ± 0.2*	2.4 ± 0.0*
N200-C164	1.2 ± 0.1	1.0 ± 0.1
N188-C164	1.2 ± 0.1	1.3 ± 0.0
Gα_z	1.1 ± 0.0	1.2 ± 0.0

HEK 293 cells were co-transfected with adenosine A₁R, pSTAT3-luc/pNFκB-luc, and pcDNA1 or the indicated Gα subunit. Transfectants were serum starved for 4 h in the presence of 100 ng/mL of PTX and then challenged with 10 μM CHA overnight. Cell lysates were analyzed for luciferase activity. Data represent the mean ± S.D. of triplicate determinations, n = 3; significant stimulations are shown in bold and italic. * CHA-stimulated protein phosphorylation is significantly greater than the basal; paired t-test, p ≤ 0.05.

ISSN: 1472-6807