B-factors analysis. Structures of the (A) WT, (B) p.Ala84Thr and (C) p.Phe128Ser ETF:QO proteins are drawn in cartoon putty representation at the β1, α1, α2, and α3 regions, where the color is ramped by residue from blue as the lowest B-factor value to red as the highest B-factor value. In addition, the size of the tube also reflects the value of the B-factor, where the larger the B-factor the thicker the tube. The structures in the other regions are colored in white and displayed in cartoon tube representation, where the size of the tube is independent of the B-factors. The red arrows indicate the positions of the mutations.