Structural and molecular basis of interaction of HCV non-structural protein 5A with human casein kinase 1α and PKR

Table 2 List of dimer interface residues in PKR and the nature of interactions between them

PKR (B chain)	PKR (C chain)	Nature of interaction
R262	D266	Ionic interaction
D266	R262	Ionic interaction
H286	C326	Hydrogen bond : NE2 of His with O of Cys
I288	Y300, V309	Hydrophobic interaction
D289	Y323	Hydrogen bond: OD2 of asp with OH of Tyr
Y293	Y323	Hydrophobic interaction
D316	H322	Ionic interaction,
V318	V318	Hydrophobic interaction
H322	D316,Y323	Hydrogen bond: ND1 of his with OD1 of Asp
		NE2 of his with OD1 of Asp
		NE2 of his with O of Tyr
		Ionic interaction
Y323	Y293, H322,D289	Hydrophobic interaction
		Hydrogen bond : O of Tyr with NE2 of His
		OH of Tyr with OD2 of Asp
N324	Y323,N324	Hydrogen bond: ND2 of Asn with O of Tyr
N324	Y323,N324	ND2 of Asn with O of Asn
C326	H286	Hydrogen bond: SG of Cys with NE2 of His

The list of non-covalent interactions at the dimeric interface of PKR[22] has been obtained by using PIC (protein interaction calculator) server[38].

ISSN: 1472-6807