Table 3 Features of non-interacting regions with substantial conformational change upon protein-protein interaction

Protein

PDB SITE record/Crystal atlas

Crystal packing

Bound

Unbound

Proteins with almost no structural change at the interface

PIP3 kinase (O)

Poor resolution and many missing residues

NI

-

HISF protein (O)

H

H

Catalytic site residues not present in NIR_PBc/IR

-

UCH-L3 (O)

Nh

Nh

Out of 4 active site residues, one in IR

NIR_PBc is near IR and are rigid in unbound form, one of the symmetry-related molecules is situated ~6Å towards this region.

Son of Sevenless (O)

H

Nh

NI

-

TGF-beta (O)

Missing residues near this region

NI

-

HPr kinase C-ter domain (E)

Resolution of PDBs is 2.8 Å

NI

-

Cystatin (E)

H

H

NI

-

DH/PH domain of TRIO (O)

Temperature factors for region under consideration abnormally high!

NI

-

Actin (O)

H

Not so high

4/12 important residues are present in NIR_PBc

Region involved in dimerization in unbound form

Alpha-1-antitrypsin (E)

H

H

Important residues present in IR

-

TGFbeta receptor (O)

Nh

Nh

3/5 of active site residues are present in NIR_PBc

-

Vitamin D binding protein (O)

Nh

Nh

NI

NIR_PBc values are close to the ones observed are IR and rigid in unbound form, one of the 4 symmetry-related molecules comes ~7Å towards this region. Another NIR_PBc value is far away from IR and is slightly mobile - the same symmetry-related molecule comes <5Å close to this region!

TolB (O)

Nh

H

NI

-

Ran GTPase (O)

Nh (nearby IR), H

H, Nh

NI

-

Sporulation response factor B (O)

Missing residues near this region

NI

-

Ran GTPase (O)

H

Nh

One important residue present in IR

-

HEW lysozyme (A)

Nh

Nh

Catalytic site residues not present in NIR_PBc/IR

-

Transferrin receptor ectodoma in (O)

Poor resolution.

Important residues not present in IR/NIR_PBc

-

Anthrax toxin receptor (O)

Missing residues near this region

NI

-

Xylanase inhibitor (E)

H

H

Catalytic site residues not present in NIR_PBc/IR

-

Fab (A)

H

Very high

NI

-

Complement C3 (O)

Nh

Nh

Important residues are not present in NIR_PBc/IR

-

Proteins with substantial structural change at interface

Arf1 GTPase (O)

Nh

Nh

-

-

Ran GTPase (O)

Nh

Nh

Important residues are present in NIR_PBC/IR

-