Structure-based sequence alignment of Bc IspF and Pf IspF. The alignment has been annotated with elements of secondary structure. Strictly conserved residues are highlighted in black. Three residues that coordinate Zn2+ are marked with a yellow star. Residues that bind CDP or CMP 1, are marked with a grey disk and those that bind CMP 2 with an orange triangle. Residues that bind MEcPP in Ec IspF  are marked with a magenta triangle. A conserved glutamate that binds either Mg2+ or Mn2+ in Ec IspF structures is highlighted with a cyan star. This figure was prepared using ALINE.