Table 2 Catalytic activities of the indicated SHP2 catalytic domain (221–524) GST fusion proteins were measured using the Malachite Green assay in the presence of different concentrations of PTP-1B peptide R-R-L-I-E-D-A-E-pY-A-A-R-G. K_m and k_cat calculated by: 1/V = (K_m/V_max) / [pY] + 1/ V_max

From: Structural insights into Noonan/LEOPARD syndrome-related mutants of protein-tyrosine phosphatase SHP2 (PTPN11)