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Table 2 Catalytic activities of the indicated SHP2 catalytic domain (221–524) GST fusion proteins were measured using the Malachite Green assay in the presence of different concentrations of PTP-1B peptide R-R-L-I-E-D-A-E-pY-A-A-R-G. Km and kcat calculated by: 1/V = (Km/Vmax) / [pY] + 1/ Vmax

From: Structural insights into Noonan/LEOPARD syndrome-related mutants of protein-tyrosine phosphatase SHP2 (PTPN11)

  kcat(s−1) Km(mM) kcat/ Km(s−1 mM−1)
WT 9.26 1.31 7.07
N308D 12.82 1.92 6.68
Y279C 0.12 9.14 0.01
Q506P 0.61 2.83 0.22