Crystal structures of the human Dysferlin inner DysF domain

BMC Structural Biology

Table 2 Aromatic arginine stack interactions in DysF domains

Stack	Dysferlin inner domain	W/R type	Interactions	Myoferlin inner domain equivalent (Residue mentioned in interaction)	Outer Dysferlin DysF equivalent
1	R1048	Aliphatic W992	Planar interaction to Y1034 in crystal contact	K1029	P1100
			H Bond to Q1010 in crystal contact
			Interacts with crystallisation phosphate
			Guanadinium Beyond NH/CZ of W992
1	W992		H bonds to crystallisation phosphate	W973	W924
1	R1046	Perpendicular W992	NH points to 6 membered ring of W992	R1027	R1096
			H bonds to crystallisation phosphate
			H bond to E951	(D392)	(A879) ?E880
2	C988		H bond to 5 membered ring of W994	C969	P920
	W994			W975	W926
	R1044	Planar W994/ Aliphatic W999	Planar stack above 5 membered ring W994	R1025	R1096
2	W999		R1044 guanadinium is beyond NE/CZ edge	W980	W930
			Aliphatic interaction with side chain of K983
2	K983		Just over 4 Å above 6 membered ring of W1042	P964	K915
2	W1042		H bond to E955	L1023 (Q936)	W1094 (E883)
3	R1040	Aliphatic W1012	H bond to E955	R1021 (Q396)	R1092 (E883)
			Guanadinium Beyond NE-CZ of W1012
3	W1012			W993	W1069
3	R1038	Planar W1012	Planar stack to 5 membered ring of W1012	R1019	R1090
		Planar W965	Planar stack to 5 membered ring of W965
3	W965		H bond to major conformation of E1031	W946 (E1012)	W894
3	R959	Aliphatic W965	Guanadinum Beyond NE-CZ of W965	R940	K887
			H bonds to major and minor conformations of E1031
Opposite Face	F954			Y935	Y882
	R1039	Planar F954	Close to planar Stack above F954	R1020	R1090
		Planar W1027	H bond to E1013	(E994)	(E1070)
			Planar Stack above 5 membered ring of W1027 closer to NE than centre
	W1027			W1008	Unclear

ISSN: 1472-6807