containing first intramolecular coiled-coil region of PcrG shows interaction with PcrV and ∆PcrV
. A. Disordered regions in PcrG predicted by PrDOS. The image was directly taken from the server. B. Proteolytic digestion of PcrG with elastase from 30 to 150 minutes. Ctrl denotes native PcrG and M is the protein molecular weight marker (10, 17, 26, 34 kDa bands from bottom to top). C. Native mass spectrometry shows the dimeric state of ∆PcrG(13–72). D. Surface plasmon resonance sensogram of ∆PcrG(13–72) and PcrV shows a 40 fold reduction in affinity of interaction on deletion of the 12 N-terminal amino acids of PcrG. E. Surface plasmon resonance sensogram of ∆PcrG(13–72) and ∆PcrV(128–294).