Figure 4From: Functional asymmetry in the lysyl-tRNA synthetase explored by molecular dynamics, free energy calculations and experimentCD studies of LysU in a variety of ligand bound states. The continuous black line (A) shows the ellipticity of 2 μM LysU without ligands; the red dashed line (B) shows 2 μM LysU + 10 mM MgCl2; the green dashed-dotted line (C) shows 2 μM LysU + 10 mM MgCl2 + 1 mM lysine; the blue dotted line (D) shows 2 μM LysU + 10 mM MgCl2 + 1 mM lysine + 250 μM AMPPCP; the brown dash dot dot line shows 2 μM LysU + 10 mM MgCl2 + 250 μM AMPPCP(E).Back to article page