Comparison of unliganded and liganded Vps25. Superposition of unliganded Vps25 (red) with Vps25 from both ESCRT-II structures [1, 2] (blue, pdb code 1U5T chain C; green, pdb code 1W7P chain B). The peptide backbones are shown as coils. Vps25 is shown in the same orientation as in figure 1A. The position of Arg83 is indicated by an arrow.