Table 1 Expression strength and solubility of the domain constructs.
Domain | Protein ID | start residue | end residue | total cellular protein | Soluble purified protein |
|---|---|---|---|---|---|
CNH | AAB48435.1 | 506/511 | 825/830 | ++ | - |
RWD | CAB52345.1 | 8/13 | 134/140 | + | - |
SEP-UBX | AAH02801.1 | 171/176 | 368 | ++ | ++ |
SEP | AAH02801.1 | 171/176 | 270/275 | ++ | ++ |
L27 | AAG34117.1 | 13 | 68/73 | ++ | ++ |
Zpr1 | AAC33514.1 | 252/257 | 416/421 | ++ | ++ |
Zpr1 | AAC33514.1 | 44/49 | 207/212 | ++ | ++ |
ZnF_C3H1 | AAF28981.1 | 126/131 | 157/162 | ++ | ++ |
G_patch | CAD38742.1 | 406/411 | 459/464 | + | + |
G_patch | AAH07871.1 | 228/233 | 279/284 | +/- | ++ |
G_patch | CAA68976.1 | 92/97 | 142/147 | + | ++ |
G_patch | AAA99715.1 | 736/741 | 787/792 | +/- | + |
OSTEO | AAA59974.1 | 14/19 | 314 | - | - |
ZnF_RBZ | CAC28312.1 | 185 | 214 | + | ++ |
PINT | AAB58732.1 | 316/321 | 404/409 | + | + |
JAB_MPN | AAD03468.1 | 54 | 191 | + | - |
JAB_MPN | AAD03465.1 | 33/38 | 172/177 | ++ | - |
DCX | AAC31696.1 | 85/90 | 181/186 | ++ | ++ |
DCX | AAC31696.1 | 85/90 | 305/310 | ++ | ++ |
DCX | AAC31696.1 | 212/217 | 305/310 | ++ | ++ |
MA3 | AAC02903.2 | 1215/1220 | 1332/1327 | ++ | - |
eIF5C | AAC02903.2 | 1487/1492 | 1579/1583 | ++ | - |
PRY | AAA36564.1 | 310/315 | 367/372 | ++ | +/- |
SPRY | AAA36564.1 | 363/368 | 493/498 | ++ | - |
TLDc | BAB13435.1 | 250/255 | 424/429 | ++ | - |