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Table 1 Expression strength and solubility of the domain constructs.

From: Fast identification of folded human protein domains expressed in E. coli suitable for structural analysis

Domain Protein ID start residue end residue total cellular protein Soluble purified protein
CNH AAB48435.1 506/511 825/830 ++ -
RWD CAB52345.1 8/13 134/140 + -
SEP-UBX AAH02801.1 171/176 368 ++ ++
SEP AAH02801.1 171/176 270/275 ++ ++
L27 AAG34117.1 13 68/73 ++ ++
Zpr1 AAC33514.1 252/257 416/421 ++ ++
Zpr1 AAC33514.1 44/49 207/212 ++ ++
ZnF_C3H1 AAF28981.1 126/131 157/162 ++ ++
G_patch CAD38742.1 406/411 459/464 + +
G_patch AAH07871.1 228/233 279/284 +/- ++
G_patch CAA68976.1 92/97 142/147 + ++
G_patch AAA99715.1 736/741 787/792 +/- +
OSTEO AAA59974.1 14/19 314 - -
ZnF_RBZ CAC28312.1 185 214 + ++
PINT AAB58732.1 316/321 404/409 + +
JAB_MPN AAD03468.1 54 191 + -
JAB_MPN AAD03465.1 33/38 172/177 ++ -
DCX AAC31696.1 85/90 181/186 ++ ++
DCX AAC31696.1 85/90 305/310 ++ ++
DCX AAC31696.1 212/217 305/310 ++ ++
MA3 AAC02903.2 1215/1220 1332/1327 ++ -
eIF5C AAC02903.2 1487/1492 1579/1583 ++ -
PRY AAA36564.1 310/315 367/372 ++ +/-
SPRY AAA36564.1 363/368 493/498 ++ -
TLDc BAB13435.1 250/255 424/429 ++ -
  1. Relative amounts of purified protein were judged from SDS-PAGE. The numbers in the columns 'start residue' and 'end residue' identify the combinations of constructs tested. Bold numbers indicate that these constructs yielded more soluble expression for the respective domain than the other constructs (see Results). '-' = no expression, '+/-' = low expression, '+' = intermediate expression, '++' = strong expression. Clones are available from the RZPD, German Resource Center for Genome Research GmbH http://www.rzpd.de, refer to supplementary table Additional file: 1.