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Table 2 Analysis of soluble constructs with 1D 1H-NMR and analytical HIC.

From: Fast identification of folded human protein domains expressed in E. coli suitable for structural analysis

Domain Protein ID start residue end residue 1H 1D-NMR analytical HIC
SEP AAH02801.1 171 270 + +
SEP AAH02801.1 176 270 + +
DCX AAC31696.1 90 181 + +/-
DCX AAC31696.1 90 186 + +
DCX AAC31696.1 85 181 + +
DCX AAC31696.1 85 186 + +
DCX AAC31696.1 212 310 - -
DCX AAC31696.1 217 310 n. d. -
DCX AAC31696.1 217 305 n. d. -
DCX AAC31696.1 85 305 +/- -
DCX AAC31696.1 90 305 +/- -
DCX AAC31696.1 90 310 - -
DCX AAC31696.1 85 310 - -
SEP-UBX AAH02801.1 171 368 + +
SEP-UBX AAH02801.1 176 368 - +/-
PINT AAB58732.1 316 409 n. d. -
PINT AAB58732.1 321 404 n. d. -
PINT AAB58732.1 316 404 n. d. -
PINT AAB58732.1 321 409 n. d. -
G_patch CAD38742.1 406 464 n. d. -
G_patch AAH07871.1 228 284 n. d. -
G_patch AAH07871.1 233 284 n. d. +/-
G_patch CAA68976.1 91 141 n. d. -
G_patch CAA68976.1 91 146 - -
G_patch CAA68976.1 96 141 - -
G_patch CAA68976.1 96 146 - +/-
G_patch AAA99715.1 736 787 n. d. -
Zpr1 AAC33514.1 252 416 n. d. -
Zpr1 AAC33514.1 257 416 n. d. -
Zpr1 AAC33514.1 44 212 n. d. -
ZnF_C3H1 AAF28981.1 131 157 n. d. -
ZnF_C3H1 AAF28981.1 126 162 n. d. -
ZnF_C3H1 AAF28981.1 131 162 - -
ZnF_RBZ CAC28312.1 185 214 n. d. -
L27 AAG34117.1 13 68 - -
L27 AAG34117.1 13 73 - -
  1. For column '1D 1H-NMR': '-' = unfolded, '+/-' = presumably folded, '+' = folded, 'n. d' = not detectable (protein amount insufficient for detection). For column 'analytical HIC': '-' = no peak upon ammoniumsulfate gradient, but protein elutes only during GdmHCl gradient, '+/-' = very small or broad peak observed upon ammoniumsulfate gradient, '+' = distinct peak observed upon ammoniumsulfate gradient.