Figure 8
From: Structural organization and interactions of transmembrane domains in tetraspanin proteins
Effect of mutations in conserved TM1 and TM2 residues on CD9 cross-linking. RD cells were transfected with constructs encoding the following fusion proteins: panel A, CD9 TM(1+2)-GFP, either wild-type or single-cysteine G80C mutant, which also carries TM1 and TM2 substitutions indicated; panel B, CD9 TM(1+2)-GFP with no cysteines or wild-type cysteines (Cys9,78,79) plus TM1 and TM2 mutations; panel C, full-length CD9-GFP with TM1 and TM2 mutations indicated. The proteins were cross-linked and analyzed by GFP Western as in Figure 7. % dimer was calculated as for Figure 7.