Figure 5
From: Deciphering structure and topology of conserved COG2042 orphan proteins
MALDI-TOF mass spectra of proteins labeled with NHS-biotin. Protein samples were mixed with various amount of NHS-biotin for 15 min at room temperature. Unlabeled protein mass spectrum is shown in grey solid line. Reagent/total lysine ratios were in Fig. 5A: 1:40 (black dotted line) and 1:20 (black dashed line), and in Fig. 5B: 1:2 (black solid line) and 2:1 (grey dotted line). The number of labeled lysines is indicated above each peak.