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Table 1 Fingerprint identification of recombinant products from pSBTN-AB31 and pSBTN-AB30 constructs.

From: Deciphering structure and topology of conserved COG2042 orphan proteins

pSBTN-AB31 constructa pSBTN-AB30 constructa Theoretical peptides
[MH]+ observed (in amu) Δmass (in ppm) [MH]+ observed (in amu) Δmass (in ppm) [MH]+ expected (in amu) Positionb (Start-End) Peptide assignment
1590.64 -31 nd c - 1590.69 3–16 GSHHHHHHGMASMK
nd c - 1050.54 -19 1050.56 17–24 VYIIDYHK
1505.71 -33 nd c - 1505.76 17–28 VYIIDYHKDDPK
1661.83 -18 1661.85 -6 1661.86 17–29 VYIIDYHKDDPKR
1601.84 -31 1601.83 -37 1601.89 49–63 GVVLDPFAQITLSNK
1844.98 -16 1845.00 -5 1845.01 49–65 GVVLDPFAQITLSNKD
2328.28 -4 2328.28 -4 2328.29 49–69 GVVLDPFA...KDKDIVR
2484.35 -16 2484.34 -20 2484.39 49–70 GVVLDPFA...KDKDIVRR
2140.06 0 2140.06 0 2140.06 71–89 IGITIVDTSWNNTSQSEFK
2296.16 0 2296.16 0 2296.16 70–89 RIGITIVDTSWNNTSQSEFK
2043.17 10 2043.18 15 2043.15 97–114 RIPILFAGNPIHYGIAYK
1887.05 0 1887.06 5 1887.05 98–114 IPILFAGNPIHYGIAYK
1244.62 -16 1244.59 -40 1244.64 143–152 WGHTFIELNK
2091.09 0 nd c - 2091.09 143–159 WGHTFIELNKELLEAYK
nd c - 865.53 69 865.47 153–159 ELLEAYK
  1. aThe two products were isolated from SDS-PAGE and treated with trypsin. The resulting peptides were analyzed by MALDI-TOF spectrometry in reflectron mode. The expected length for the two products are: 180 amino acids for SSO0551 with the 6His-tag at the N terminus (pSBTN-AB31 construct) and 222 amino acids for SSO0551 extended version (42 additional amino acids at the N terminus and a 6His-tag; pSBTN-AB30 construct).
  2. bPeptide positions refers to SSO0551.
  3. c«nd» denote the peptides not detected in one of the samples.