Table 1 Fingerprint identification of recombinant products from pSBTN-AB31 and pSBTN-AB30 constructs.
From: Deciphering structure and topology of conserved COG2042 orphan proteins
pSBTN-AB31 constructa | pSBTN-AB30 constructa | Theoretical peptides | ||||
|---|---|---|---|---|---|---|
[MH]+ observed (in amu) | Δmass (in ppm) | [MH]+ observed (in amu) | Δmass (in ppm) | [MH]+ expected (in amu) | Positionb (Start-End) | Peptide assignment |
1590.64 | -31 | nd c | - | 1590.69 | 3–16 | GSHHHHHHGMASMK |
nd c | - | 1050.54 | -19 | 1050.56 | 17–24 | VYIIDYHK |
1505.71 | -33 | nd c | - | 1505.76 | 17–28 | VYIIDYHKDDPK |
1661.83 | -18 | 1661.85 | -6 | 1661.86 | 17–29 | VYIIDYHKDDPKR |
1601.84 | -31 | 1601.83 | -37 | 1601.89 | 49–63 | GVVLDPFAQITLSNK |
1844.98 | -16 | 1845.00 | -5 | 1845.01 | 49–65 | GVVLDPFAQITLSNKD |
2328.28 | -4 | 2328.28 | -4 | 2328.29 | 49–69 | GVVLDPFA...KDKDIVR |
2484.35 | -16 | 2484.34 | -20 | 2484.39 | 49–70 | GVVLDPFA...KDKDIVRR |
2140.06 | 0 | 2140.06 | 0 | 2140.06 | 71–89 | IGITIVDTSWNNTSQSEFK |
2296.16 | 0 | 2296.16 | 0 | 2296.16 | 70–89 | RIGITIVDTSWNNTSQSEFK |
2043.17 | 10 | 2043.18 | 15 | 2043.15 | 97–114 | RIPILFAGNPIHYGIAYK |
1887.05 | 0 | 1887.06 | 5 | 1887.05 | 98–114 | IPILFAGNPIHYGIAYK |
1244.62 | -16 | 1244.59 | -40 | 1244.64 | 143–152 | WGHTFIELNK |
2091.09 | 0 | nd c | - | 2091.09 | 143–159 | WGHTFIELNKELLEAYK |
nd c | - | 865.53 | 69 | 865.47 | 153–159 | ELLEAYK |