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Table 2 Monoisotopic [M+H]+ peptides generated by various proteases after NHS-biotin labeling of SSO0551.

From: Deciphering structure and topology of conserved COG2042 orphan proteins

Protease [M+H]+ (observed) [M+H]+ (expected) Δmass (ppm) Position (Start-End) Peptide assignmenta NHS-biotin labelb Modified residuesa
Trypsin 1731.94 1731.84 -57 3–14 VYIIDYHK DDPK 1 K 10
Trypsin 2114.27 2114.02 118 3–15 VYIIDYHK DDPK R 2 K10andK14
Trypsin 1888.15 1887.94 111 3–15 VYIIDYHKDDPKR 1 K10orK14
Trypsin 1991.21 1990.98 -119 1–14 MKVYIIDYHKDDPK 1 K2orK10
Trypsin 2147.33 2147.08 -120 1–15 MKVYIIDYHKDDPKR 1 K2orK10orK14
Trypsin 2163.33 2163.07 -120 1–15 MoxyKVYIIDYHKDDPKR 1 K2orK10orK14
Trypsin 2373.41 2373.16 -107 1–15 MKVYIIDYHKDDPKR 2 K2or/andK10or/andK14
Trypsin 1180.77 1180.69 -64 20–25 K LVK LK 2 K20andK23
Trypsin 1203.76 1203.66 -87 24–31 LK IAEFTR 1 K 25
Trypsin 1770.16 1769.98 102 21–31 LVK LK IAEFTR 2 K23andK25
Trypsin 1898.15 1898.08 -39 20–31 KLVKLKIAEFTR 2 K20orK23orK25
Trypsin 1127.68 1127.60 -71 50–56 DK DIVRR 1 K 51
Trypsin 3064.63 3064.63 1 32–55 VGKGVVL...NKDKDIVR 2 K34or/andK49or/andK51
Trypsin 2554.57 2554.37 -78 35–55 GVVL...NKDKDIVR 1 K49orK51
Trypsin 2710.61 2710.47 -51 35–56 GVVL...NKDKDIVRR 1 K49orK51
Trypsin 2838.65 2838.55 -34 32–55 VGKGVVL...NKDKDIVR 1 K34orK49orK51
Arg-C 2838.75 2838.55 -69 32–55 VGKGVVL...NKDKDIVR 1 K34orK49orK51
Trypsin 2749.36 2749.36 0 57–78 IGIT...SEFK NIR 1 K 75
Arg-C 2749.60 2749.36 -87 57–78 IGIT...SEFK NIR 1 K 75
Glu-C 1089.56 1089.55 -8 74–80 FK NIRGE 1 K 75
Trypsin 2111.22 2111.11 -52 123–138 LSNVVKWGHTFIELNK 1 K 128
Glu-C 2068.19 2068.10 -41 120–135 AIKLSNVVKWGHTFIE 1 K122orK128
Trypsin 1330.74 1330.67 -55 146–154 NKTEEDIKK 1 K147orK153
Glu-C 1256.65 1256.67 13 151–158 DIKKIERE 1 K153orK154
Trypsin 1486.85 1486.76 -60 148–157 TEEDIKKIEREIIEK 1 K153orK154
Trypsin 1383.76 1383.77 5 154–162 K IEREIIEK 1 K 154
Trypsin 1867.14 1867.07 -36 154–166 KIEREIIEKILEK 1 K162orK154
Trypsin 1340.71 1340.75 29 158–166 EIIEKILEK 1 K162orK166
Trypsin 1739.01 1738.98 -19 155–166 IEREIIEKILEK 1 K162orK166
  1. aUnequivocal modified residues are indicated in bold characters.
  2. bNHS-biotin label (+226.08 amu).