Table 2 Monoisotopic [M+H]+ peptides generated by various proteases after NHS-biotin labeling of SSO0551.
From: Deciphering structure and topology of conserved COG2042 orphan proteins
Protease | [M+H]+ (observed) | [M+H]+ (expected) | Δmass (ppm) | Position (Start-End) | Peptide assignmenta | NHS-biotin labelb | Modified residuesa |
|---|---|---|---|---|---|---|---|
Trypsin | 1731.94 | 1731.84 | -57 | 3–14 | VYIIDYHK DDPK | 1 | K 10 |
Trypsin | 2114.27 | 2114.02 | 118 | 3–15 | VYIIDYHK DDPK R | 2 | K10andK14 |
Trypsin | 1888.15 | 1887.94 | 111 | 3–15 | VYIIDYHKDDPKR | 1 | K10orK14 |
Trypsin | 1991.21 | 1990.98 | -119 | 1–14 | MKVYIIDYHKDDPK | 1 | K2orK10 |
Trypsin | 2147.33 | 2147.08 | -120 | 1–15 | MKVYIIDYHKDDPKR | 1 | K2orK10orK14 |
Trypsin | 2163.33 | 2163.07 | -120 | 1–15 | MoxyKVYIIDYHKDDPKR | 1 | K2orK10orK14 |
Trypsin | 2373.41 | 2373.16 | -107 | 1–15 | MKVYIIDYHKDDPKR | 2 | K2or/andK10or/andK14 |
Trypsin | 1180.77 | 1180.69 | -64 | 20–25 | K LVK LK | 2 | K20andK23 |
Trypsin | 1203.76 | 1203.66 | -87 | 24–31 | LK IAEFTR | 1 | K 25 |
Trypsin | 1770.16 | 1769.98 | 102 | 21–31 | LVK LK IAEFTR | 2 | K23andK25 |
Trypsin | 1898.15 | 1898.08 | -39 | 20–31 | KLVKLKIAEFTR | 2 | K20orK23orK25 |
Trypsin | 1127.68 | 1127.60 | -71 | 50–56 | DK DIVRR | 1 | K 51 |
Trypsin | 3064.63 | 3064.63 | 1 | 32–55 | VGKGVVL...NKDKDIVR | 2 | K34or/andK49or/andK51 |
Trypsin | 2554.57 | 2554.37 | -78 | 35–55 | GVVL...NKDKDIVR | 1 | K49orK51 |
Trypsin | 2710.61 | 2710.47 | -51 | 35–56 | GVVL...NKDKDIVRR | 1 | K49orK51 |
Trypsin | 2838.65 | 2838.55 | -34 | 32–55 | VGKGVVL...NKDKDIVR | 1 | K34orK49orK51 |
Arg-C | 2838.75 | 2838.55 | -69 | 32–55 | VGKGVVL...NKDKDIVR | 1 | K34orK49orK51 |
Trypsin | 2749.36 | 2749.36 | 0 | 57–78 | IGIT...SEFK NIR | 1 | K 75 |
Arg-C | 2749.60 | 2749.36 | -87 | 57–78 | IGIT...SEFK NIR | 1 | K 75 |
Glu-C | 1089.56 | 1089.55 | -8 | 74–80 | FK NIRGE | 1 | K 75 |
Trypsin | 2111.22 | 2111.11 | -52 | 123–138 | LSNVVKWGHTFIELNK | 1 | K 128 |
Glu-C | 2068.19 | 2068.10 | -41 | 120–135 | AIKLSNVVKWGHTFIE | 1 | K122orK128 |
Trypsin | 1330.74 | 1330.67 | -55 | 146–154 | NKTEEDIKK | 1 | K147orK153 |
Glu-C | 1256.65 | 1256.67 | 13 | 151–158 | DIKKIERE | 1 | K153orK154 |
Trypsin | 1486.85 | 1486.76 | -60 | 148–157 | TEEDIKKIEREIIEK | 1 | K153orK154 |
Trypsin | 1383.76 | 1383.77 | 5 | 154–162 | K IEREIIEK | 1 | K 154 |
Trypsin | 1867.14 | 1867.07 | -36 | 154–166 | KIEREIIEKILEK | 1 | K162orK154 |
Trypsin | 1340.71 | 1340.75 | 29 | 158–166 | EIIEKILEK | 1 | K162orK166 |
Trypsin | 1739.01 | 1738.98 | -19 | 155–166 | IEREIIEKILEK | 1 | K162orK166 |