Figure 6

FSC between 2 independent reconstruction for each method. Green and blue lines show the correlation as function of the spatial frequency using 300 particles from the Focal pair method and HWPM respectively. Using the 0.5 correlation point criterion to assess the resolution of the 3D structure, the dotted line gives a resolution of 32 Å and thin line gives a resolution of 24 Å. The purple line shows a reconstruction using 500 particles where the orientation is obtained from the HWPM method the estimated resolution using 0.5 correlation point shows 14.5Å. The red line shows the 2 times expected FSC for Gaussian noise.