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Figure 2 | BMC Structural Biology

Figure 2

From: Crystal structures of an Extracytoplasmic Solute Receptor from a TRAP transporter in its open and closed forms reveal a helix-swapped dimer requiring a cation for α-keto acid binding

Figure 2

Oligomeric state of TakP in solution. A: Elution profile of a gel filtration experiment with TakP in 50 mM NaCl, 20 mM Tris HCl pH 8.0. A standard curve is superimposed and was calculated using the known molecular weight of four protein standards (blue circles). The theoretical molecular weight of TakP is 39 kDa. B: Denaturing gel electrophoresis before (Control) and after 3 hours incubation with the cross-linker glutaraldehyde (50 mM). Two concentrations of TakP were used as indicated. Each lane contains 2 μg of protein.

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BMC Structural Biology

ISSN: 1472-6807

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