Mechanisms for stabilisation and the maintenance of solubility in proteins from thermophiles

BMC Structural Biology

Table 1 Summary of calculated properties.

Fig	Set	Property	50% cumulative value ^a			t-test statistic ^b
			Meso	modT	hyperT	M-mT	mT-hT	M-hT
2a	291	GminN	-0.225	-0.244	-0.300	1.04	4.73	7.12
2b	67	GminN	-0.197	-0.221	-0.305	2.03	3.31	5.31
3a	291	StotalN	-0.971	-0.940	-1.086	0.63	7.83	9.14
3b	67	StotalN	-0.950	-0.945	-1.168	0.98	5.79	5.33
5	291	SdiffN (0.8 VdW tol)	-0.616	-0.606	-0.613	0.31	1.03	0.94
5	291	SdiffN (1.2 VdW tol)	-0.334	-0.336	-0.326	0.97	1.95	1.34
6a	291	Contacts per atom	6.000	6.009	5.945	2.43	1.95	2.06
6b	291	Contact Order	0.301	0.342	0.322	4.09	2.22	1.92
7a	291	Born Energy (Asp)	20.19	20.27	19.72	1.27	1.09	0.03
7b	291	Born Energy (Glu)	15.47	13.57	13.87	1.51	1.53	4.29
7c	291	Born Energy (All)	15.83	14.65	13.97	1.40	2.40	5.10
8b	291	Polar ASA/Charged ASA	0.696	0.506	0.428	1.08	5.77	12.26
8c	291	Non-charged patch size	358.2	276.0	186.8	2.29	4.90	8.59
9a	291	Triple product	19.23	19.14	31.27	1.29	7.99	10.89
9b	67	Triple product	15.98	17.95	31.31	1.14	4.90	6.36

a. Values at 50% level of cumulative distributions for mesophile (Meso, M), moderate thermophile (modT, mT), and hyperthermophile (hyperT, hT)proteins.
b. Student's t-tests are employed for the 3 possible pairings, for each property/dataset. These are applied to the underlying populations of each property, and given in bold where they are different at the 95% confidence level.

ISSN: 1472-6807