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Table 2 Comparison of contact distances (in Angstroms) in binding sites of the PA-IIL mutants

From: Engineering of PA-IIL lectin from Pseudomonas aeruginosa – Unravelling the role of the specificity loop for sugar preference

 

PA-IIL G24N

PA-IIL S22A

PA-IIL S23A

 

Me-β-D-Man

Me-α-L-Fuc

Me-β-D-Man

Me-α-L-Fuc

Me-α-L-Fuc

Man (O6) -- Ser23 (OG)

2.80

-

3.50

-

-

Man (O6) -- Asn24 (ND2)

3.31

--

-

-

-

Man (O6) -- Asp96 (OD2)

-

-

2.67

-

-

Man (O6) -- Ser23 (N)

-

-

3.20

-

-

Man (O6) -- Gly24 (N)

-

-

2.94

-

-

O5--Ser22 (OG)

-

3.44

-

-

3.44

O5--Ser23 (OG)

3.20

3.19

3.19

3.35

-

Asp96 (OD2) -- Ser22 (OG)

2.71

2.78

-

-

2.70

Asp96 (OD2) -- H2O (2)

-

-

-

2.60

-

H2O (2) -- Ser23 (OG)

-

-

-

-

-

Fuc (O1) -- H2O (1)

-

3.08

-

3.19

3.03

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BMC Structural Biology

ISSN: 1472-6807

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