Figure 4

DSC transitions of muscle fibres in rigor (dashed line) and in the presence of ADP (solid line). MOPS buffer was used to minimize the effect of pH change due to increasing temperature. 4 mM MgADP was added to rigor buffer before measurement. In order to demonstrate the effect of MgADP on fibres, the DSC traces of myosin rod and the population of actin at about 64°C were subtracted (dotted line).