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Table 1 DSC results on muscle fibres: transition temperatures in range of 40 – 85°C. Glycerinated muscle fibres prepared from psoas muscle of rabbit were measured in rigor, strongly and weakly binding state of myosin to actin. The transition temperatures were derived from the original DSC thermograms

From: Differential scanning calorimetry study of glycerinated rabbit psoas muscle fibres in intermediate state of ATP hydrolysis

Experimental results

T1 (°C)

T2 (°C)

T3 (°C)

T4 (°C)

Rigor

52.2

59.2

-

66.1

ADP

53.0

57.8

-

67.8

AMP.PNP

52.7

57.7

62.1

70.4

PPi

52.8

57.5

-

65.8

ADP.Vi

53.6

57.8

63.3

68.5

ADP.AlF4

52.9

57.9

64.4

74.7

ADP.BeFx

53.2

58.4

63.8

77.8

  1. Mean values: Tm1 = 52.9 ± 0.5°C (n = 9)
  2. Tm2 = 57.9 ± 0.5°C (n = 9)
  3. Tm3 = 63.7 ± 0.7°C (n = 7)