Figure 6

Dimerization of SuhB is induced by Mg²⁺, or Mg²⁺ and Li⁺, but not by Li⁺ alone. Traces of the sedimentation coefficient distribution recorded in sedimentation velocity experiments of M. tuberculosis SuhB. SuhB was at 1.0 mg.ml^-1 in 20 mM Tris-HCl pH 7.9, 50 mM NaCl, plus MgCl₂ or LiCl as indicated. Samples were centrifuged at 40,000 rpm at 4°C for at least 12 hours. The peak at 1.6 S corresponds to a molecular weight of 30,147 Da, the peak at 2.5 S corresponds to 50,116 Da.