Figure 6

NMR titrations of Brd2 BD2 with mutated histone H4 tails. Brd2 BD2 was titrated with different H4-AcK12 peptide mutants, including H4-L10A, H4-L10G, H4-A15G, H4-acid and the unacetylated peptide H4-U. Combined chemical shift perturbation was calculated using the equation, $\Delta {\delta }_{ppm}=\sqrt{{(\Delta {\delta }_{HN})}^2+{(\Delta {\delta }_N{\alpha }_N)}^2}$, and R = [peptide]/[bromodomain].