Figure 2

The phosphate binding pocket of the Grb7 SH2 domain. (a) Electrostatic potential surface of Grb7 SH2 generated using CCP4MG [80]. Positively charged electrostatic potential is coloured blue and negatively charged electrostatic potential is coloured red. The positions of the phosphate binding pocket is indicated. (b) A 2F_o - F_c electron density map depicting the phosphate binding pocket of Grb7 SH2. A sulphate ion co-crystallised in this pocket in all four molecules in the asymmetric unit. The map is contoured at 1 σ. R438, R458, Q461 and S460 form direct contacts with the sulphate ion and are labeled. The side-chain of R462 lacks well defined density and is probably fairly mobile in the crystal.