Figure 7

Model of the Grb7 SH2/G7-18NATE complex. (a) The modeled structure of the complex. The Grb7 SH2 domain is shown in grey in cartoon representation and G7-18NATE in stick representation with carbon atoms coloured green. The positions of the two α-helices are indicated. The model was prepared using the structure of the Grb2 SH2:pYVN complex (1JYR; [52]) as a template and energy minimised using NAMD [79]. (b) A close up of the Grb7 SH2/G718-NATE interface. The Glu3 and Tyr5 side-chains occupy the phosphate binding pocket while the Asn7 side-chain occupies a second pocket in the specificity determining region of the domain. Only Glu3, Tyr5, Asp6 and Asn7 are shown for clarity.