Figure 7
Biological activities of specificity-altered BMP-2 variants. (a) The BMP variants L100K/N102D (red) and S85R/A86P (green) exhibit a two- to three-fold lower EC50 value for ALP induction as wildtype BMP-2. The increased biological activity correlates with the increased affinity for ActR-IIB/ActR-II (L100K/N102D) or BMPR-II (S85R/A86P). (b) The single variant BMP-2 L100K, which has a six-fold higher affinity for ActR-IIB, shows no increased biological activity in C2C12 cells.