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Table 1 Mutational analysis of the ActR-IIB interface (Biosensor analysis)

From: A silent H-bond can be mutationally activated for high-affinity interaction of BMP-2 and activin type IIB receptor

 

Ligand proteins (immobilized)

ActR-IIBECD

BMP-2a

BMP-7

Act-A

 

rel. KD (app. KD in nM)

wildtype

1 (7700)

1 (3600)

1 (65)

E34A

1

1.8

1

K37A

6.1

0.3

0.7

Y42A

61

49

290

R46A

8.4

3.3

2.9

K56A

11

14

2.7

W60A

n.bb

n.b.

n.b.

L61A

6.4

6.4

24

L61P

7.9

19

2510

D63A

9.6

6.7

8.6

Q80A

1.3

1

1.1

F83Ac

n.b.

n.b.

n.b.

  1. a) For these measurements glycosylated BMP-2 expressed in CHO cells was used.
  2. b) Binding is below detection limit (KD > 1 mM.)
  3. c) This variant might be not native and exhibits an altered electrophoretical mobility in SDS-PAGE under non-reducing conditions.
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BMC Structural Biology

ISSN: 1472-6807

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