Figure 8
From: Metal ion-dependent, reversible, protein filament formation by designed beta-roll polypeptides
Negative stained EM images of BioBRD2 and BRD2 in lanthanum solution. A) BioBRD2 alone; B) 2:1 mixture of BRD2:BioBRD2; C) 20:1 mixture of BRD2:BioBRD 2; D) 40:1 mixture of BRD2:BioBRD2. These images at 30,000 × magnification on Formvar-coated nickel grids show the inverse relationship between the concentration of biotinylated BRD2 and the length of filaments formed. BioBRD2 does not form filaments in lanthanum solutions and it reduces the length and abundance of BRD2 filaments compared to identical conditions with BRD2 alone.