Figure 3

Effect of GSH on oligomeric status of human GST. SEC profile of human GST. Curve "a" represent h GST incubated and run with buffer containing 2 mM GSH, while curve "b" represent h GST incubated and run with buffer not containing GSH. The curves have been displaced on Y-axis for presentation. Inset shows the SDS-PAGE profile of glutaraldehyde cross-linked h GST protein samples. Lanes 1–4 represent molecular weight markers, uncross-linked native h GST, glutaraldehyde cross-linked h GST incubated with GSH and h GST incubated without GSH protein samples, respectively.