Figure 4

Deciphering the reversibility of the tetramer-dimer transition. A. SEC profile of Pf GST. Curves a-e represent tetrameric Pf GST, dimeric Pf GST, dimeric Pf GST dialysed for 4 h in buffer devoid of GSH, dimeric Pf GST dialysed for 24 h in buffer devoid of GSH, dimeric Pf GST loaded on the column equilibrated and run with buffer without GSH. The curves have been displaced on Y-axis for presentation. B. Enzymatic activity assay of the eluted peaks. In the figure, bars a-e represents data for the peaks described in 4A.