Figure 2

Electron density maps of the RTA-NMU complex instereo. (A) Initial 2mFo-DFc map before repositioning of Tyr80. Contoured at 1σ (blue), 2σ (purple), 3σ (pink), 4σ (magenta), and 5σ (red). (B) Density recalculated after removal of the Tyr80 ring. (C) After repositioning Tyr80 to fit the observed density and one round of refinement. (D) Density before placement of N-methylurea and water molecules at the active site. (E) Final structure after placement of ligand and water.