NMR spectroscopic and analytical ultracentrifuge analysis of membrane protein detergent complexes

BMC Structural Biology

Table 1 General properties of detergents used.

Detergent	MW of monomer [Da]	cMc [mM] ^a	Aggregation number ^a	Theoretical ${\bar{v}}_{d}$ ^b	Concentration used in extraction buffer [mM]	References
Zwitterionic
Zw-3.14 ^c	363.6	0.16	83–130 (130 ^d)	0.9714	14.5	[24, 25]
Zw-3.12	335.0	2.8 ^e	55–87 (80 ^d)	0.9568	15.3	[25, 26]
LDAO	229.4	1–2	76	1.0597	16.0	[27]
FC14	379.5	0.12	80–120 ^f (100 ^d)	0.9876	5.3	[8, 24]
FC12, DPC	351.5	1.5	50–60	0.9747	-	[24, 25]
Anionic
SDS	288.4	2.6 ^g	62–101 (100 ^d)	0.8578	17.5	[24, 28]
DPh	373.4			0.8123	-	[24]
Non-ionic
Brij-35	1198 av.	0.091	40		4.3	[25, 29]
C12E8	538.8	0.09 ^g	90–120 ^h (100 ^d)		9.6	[25]
Triton X100	647 av.	0.23	75–165 (160 ^d)		16.0	[25, 30]
DM	482.6	1.8	69	0.7725	12.0	[24, 31]
DDM	510.6	0.17	78–149	0.7932	-	[24, 28]
NG	306.4	6.5		0.8495	-	[24]
OG	292.4	18–20	27–100	0.8352	-	[32]

^a – in H₂O, if otherwise is not specified; ^b – from [18] or calculated according to [18]; ^c – detergents used for extraction screen are in bold font; ^d – averaged values used in calculations; ^e – 20 mM Tris-HCl, pH 8.0, 100 mM NaCl; ^f – estimated value; ^g – pH 7.5; ^h – 10 mM TES, pH 7.5, 50 mM NaCl, 0.1 mM CaCl₂

ISSN: 1472-6807