Distribution of the number of residues in segments with particular N
. Different colored curves show distributions obtained for different minimal length (F
) of resulting segments. The most dramatic changes occur when the value changes from 2 to 3 residues, clearly identifying the threshold for noise discrimination. The distributions are almost the same for values 3–6 of F
, while for higher values, the distributions start to deviate from each other (marked as regions of differences), thus identifying the threshold for clear signal separation. Therefore, the optimal value of F
for analysis should be between 3 and 6 because here is where the noise is eliminated without significant affect on the signal.