Example of non-sequential alignment with reverse segment. Structures of adoment dependent methyltransferase (PDB-code 1i9g:A) and zeta-crystallin (PDB-code 1yb5:A) have been aligned by TOPOFIT with N
/RMSD of 115/1.7 Å. The longest sequential alignment is colored in blue. The fragment aligned in reverse order is colored in orange. The right side of the picture displays the corresponding alignment plot.