Figure 3

Comparison between NOE crosspeaks from hybrid and parental Cp and Pf A2K rubredoxins. Hybrid rubredoxin NOE crosspeaks between protons assigned to a common parent are compared to the corresponding parental peaks. Normalized volumes (panel A) and heights (panel B) from the 3D ¹H-¹⁵N-¹H 3D NOESY data are given for 1045 pairs of hybrid and parental NOE peaks, while panel C provides analogous peak height data from 2D ¹⁵N suppressed ¹H-¹H NOESY experiments (365 NOE pairs). The hybrid-parent type for each pair of NOE peaks is indicated in red (metal binding site-swapped Pf A2K and Pf A2K), magenta (metal binding site-swapped Cp and Pf A2K), light blue (metal binding site-swapped Pf A2K and Cp), and dark blue (metal binding site-swapped Cp and Cp). Individual symbols denote NOE crosspeaks involving Lys 2 H^N (◆), Thr 28 H^γ1 (▲), Phe 49 H^δ (▼) and sequential H^α-H^N connectivities for conformationally extended residues (■). The intraresidue Trp 37 H^ε3-H^ζ3 and Trp 37 H^δ1-H^ε1 crosspeaks were used as intensity references for the 2D and 3D NOE data, respectively.