Table 2 Thermodynamic parameters from ITC of the MBP peptide binding to CaM. The shown values are from fitting for the specific binding site only. In parentheses, the values for the first binding event are given when fitting is done to a 4-site model.
T | Kd (μM) | ΔH (kcal/mol) | -T ΔS (kcal/mol) | n |
|---|---|---|---|---|
298.15 K (25°C) | 3.4 (1.0) | -1.47 ± 0.13 (-1.44 ± 0.04) | -6.00 (-6.73) | 0.83 ± 0.23 (1) |
303.15 K (30°C) | 2.3 (1.1) | -2.11 ± 0.06 (-2.07 ± 0.02) | -5.71 (-6.18) | 0.67 ± 0.02 (1) |
308.15 K (35°C) | 1.9 (1.0) | -2.38 ± 0.10 (-2.34 ± 0.03) | -5.67 (-6.13) | 0.63 ± 0.02 (1) |
313.15 K (40°C) | 25.9 (1.7) | -1.09 ± 0.97 (-0.73 ± 0.05) | -5.48 (-7.56) | 0.70 ± 0.17 (1) |