Figure 3

PI binding pocket. a) Ribbon representation of the structures A (cyan) and B (silver) with the (2Fo-Fc) electron density for the side chain of Met1489 residue in structure A. The position of a bound-sulphate in the 2IWL structure is depicted for comparison. b) View of ligand-binding pocket in structure B, showing glycerol modelled into 2Fo-Fc electron density that is central to the proposed phosphoinositide binding site. Structure B is shown as a stick model and coloured based on atom types.