Figure 2

Multiple structural alignment of TPK sequences. Mouse in blue (PDB id 2F17), Candida albicans SC5314 in red (Swiss-Prot id CA1462), Saccharomyces cerevisiae in green (PDB id 1IG0), Human (Swiss-Prot id Q9H3S4), Bovine (Swiss-Prot id Q5E9T4), Caenorhabditis elegans (Swiss-Prot id P30636), Bacillus subtilis (Swiss-Prot id O34664], Geobacillus kaustophilus (Swiss-Prot id Q5L0R6), Bacillus cereus (strain ZK/E33L) (Swiss-Prot id Q636G7), Bacillus thuringiensis subsp. Konkukian (Swiss-Prot id Q6HEV6), Bacillus cereus (strain ATCC 14579/DSM 31) (Swiss-Prot id Q819U8), Staphylococcus aureus (strain COL) (Swiss-Prot id Q5HGL0), Streptococcus pyogenes serotype M6 (Swiss-Prot id Q5XDX1),Enterococcus faecalis (Swiss-Prot id Q82ZE3), Clostridium tetani (Swiss-Prot id Q895P3), Fusobacterium nucleatum subsp. vincentii ATCC 49256 (Swiss-Prot id Q7P831), Fusobacterium nucleatum subsp. nucleatum (Swiss-Prot id Q8RF31), Thermoanaerobacter tengcongensis (Swiss-Prot id Q8R9T9), Francisella tularensis subsp. Tularensis (Swiss-Prot id Q5NFC3), Silicibacter pomeroyi (Swiss-Prot id Q5LTU1), Porphyromonas gingivalis (Swiss-Prot id Q7MTP9), Symbiobacterium thermophilum (Swiss-Prot id Q67QK6), Brucella melitensis (Swiss-Prot id Q8YJ01). Secondary structures are numbered as in C. albicans. Strictly conserved residues are boxed with a thin blue line. C. albicans TPK residues making contacts with Mg²⁺ ions are highlighted in brown, those in contact with phosphate and Mg²⁺ in green, those in contact with thiamine or thiamine-PNP in pink, and those in contact with thiamine or thiamine-PNP and Mg²⁺ in purple. The residues exclusively interacting with thiamine-PNP (and not thiamine) are in grey, and those in contact both with thiamine-PNP and phosphate are in light blue.