Figure 1From: Ligand-induced conformational changes in a thermophilic ribose-binding proteinExpression and purification of the tm0958 ORF. (A) Gel-filtration (Superdex S75) chromatogram of the immobilized metal affinity purified tmRBP. Fractions (10 mL) and the void volume of the S75 column (Vo) are indicated. (B) SDS-PAGE of column fractions. Lane 1 is a molecular mass ladder.Back to article page