Figure 1

Amino acid sequence and predicted secondary structure for human mesothelin. The first line gives the residue serial numbers and the second the sequence. The mature mesothelin sequence starts from the residue number 1, after the furin cleavage at the ARG-ARG (rr) sequence of the precursor. The signalling sequence for the GPI attachment that was suggested in an earlier study [1] is shown in low-case italics; the signalling sequence predicted using a current prediction program [45] is colored green. The 9 lines that follow give the predicted secondary structural type for each residue. The names of the programs are indicated at the right-hand side. The blue color indicates residues that were predicted to be helical unanimously by all programs. The magenta indicates all other helical predictions. The last line gives the secondary structures of model#1 of the I-TASSER server, calculated using the DSSP program (H: Helix, program output states H, h, G; E: Beta, E; C: Coil, all other output states). The helical residues in this model are colored green. The vertical lines indicate the boundaries of the 8 repeats of model#1 of the I-TASSER server.