Table 3 Summary of pseudoactivated MK2 construct expression, enzymatic activity, and crystallization.
From: Rational mutagenesis to support structure-based drug design: MAPKAP kinase 2 as a case study
Construct | Yield (mg/L) | Enzymatic Activity (cts/nM) | Crystallography | |||
|---|---|---|---|---|---|---|
| Â | Crude | Final | Without p38 activation | With p38 activation | Crystals Obtained? | Diffraction? |
MK2(41–364, T222E) | 3.0 | 1.0 | 0.30 | 0.34 | Yes | No |
MK2(47–366, T222E) | 6.0 | 4.0 | <0.02 | <0.02 | Yes | Yes |
MK2(47–366, T334E) | 4.5 | 2.0 | 1.5 | 1.2 | Yes | No |